Translational_Unit

Part:BBa_K4773009:Design

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-10-01)


RBS-AprBA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1476
    Illegal XhoI site found at 2596
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1813
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The RBS (BBa_B0034) was chosen because it has a high translation initiation rate and is compatible with most bacterial hosts.


Source

This part is derived from the genomic sequence of Clostridium acetobutylicum, a gram-positive anaerobic bacterium that can produce acetone, butanol and ethanol from various substrates.

References